Re: KPL's True Blue - fading?

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From:Geoff McAuliffe <mcauliff@UMDNJ.EDU>
To:vedran lovic <>
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vedran lovic wrote:

> Hi everyone,
>           Several months ago we've used KPL's True Blue instead of DAB in an
> immunocytochemistry (ICC) run for the first time. We were staining GnRH
> neurons in bird brains.  At the time the procedure worked fine, but I just
> went back to quantify some neurons in these same brains and it seems that
> there has been a drastic fading of True Blue neuronal staining.  Does anyone
> have any experience with True Blue?  Any input would be greatly appreciated.
>   Thanx.
> Ved Lovic
> University of Toronto at Mississauga
> 3359 Mississauga Rd. N. #1029e
> Mississauga, ON
> L5L 1C6
> (905)828-5297

    My experience is that DAB, even with intensification with nickel and cobalt,
will fade if Permount is the coverslip mounting medium. I don't know about True
Blue. I now use only DPX to mount coverslips.

Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029

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