I find this interesting, as i had a a problem with X-gal dissolving in
DPX when i used it to mount the coverslips. I use a glycerol based
mountant now, which, whilst it isnt perfect, does mean that i can still
see my stainng 1 year on.....

emma carter
> -----Original Message-----
> From:	hborgeri@wfubmc.edu [SMTP:hborgeri@wfubmc.edu]
> Sent:	Monday, July 10, 2000 3:32 PM
> To:	Geoff McAuliffe
> Cc:	histonet
> Subject:	Re: KPL's True Blue - fading?
> 
> Geoff,
> 
> Where do you purchase DPX and would you be able to supply a catalog
> number and cost?
> 
> Thanks,
> 
> Hermina Borgerink
> Wake Forest University
> Department of Pathology
> Winston-Salem, NC
> 
> Geoff McAuliffe wrote:
> > 
> > vedran lovic wrote:
> > 
> > > Hi everyone,
> > >           Several months ago we've used KPL's True Blue instead of
> DAB in an
> > > immunocytochemistry (ICC) run for the first time. We were staining
> GnRH
> > > neurons in bird brains.  At the time the procedure worked fine,
> but I just
> > > went back to quantify some neurons in these same brains and it
> seems that
> > > there has been a drastic fading of True Blue neuronal staining.
> Does anyone
> > > have any experience with True Blue?  Any input would be greatly
> appreciated.
> > >   Thanx.
> > >
> > > Ved Lovic
> > > University of Toronto at Mississauga
> > > 3359 Mississauga Rd. N. #1029e
> > > Mississauga, ON
> > > L5L 1C6
> > > (905)828-5297
> > > vedranl@hotmail.com
> > >
> > 
> >     My experience is that DAB, even with intensification with nickel
> and cobalt,
> > will fade if Permount is the coverslip mounting medium. I don't know
> about True
> > Blue. I now use only DPX to mount coverslips.
> > 
> > Geoff
> > --
> > **********************************************
> > Geoff McAuliffe, Ph.D.
> > Neuroscience and Cell Biology
> > Robert Wood Johnson Medical School
> > 675 Hoes Lane, Piscataway, NJ 08854
> > voice: (732)-235-4583; fax: -4029
> > mcauliff@umdnj.edu
> > **********************************************