Gelatin/Chrome Alum for bone
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From: | Mary Stevens <Mstevens@genetics.com> |
To: | histonet@pathology.swmed.edu |
Reply-To: | |
Content-Type: | text/plain; charset=US-ASCII |
Hi all,
For years, I've been using the same Gel-Chrome Alum Concentrations/methods to coat slides for undecalcified bone (for MMA) - lately we are getting an overall background staining on the slides (not clumping, but what appears as too much gelatin.) The obvious thought is to decrease the 1% to 0.75% gelatin - but I'd like to hear what solutions you are using and your thoughts on why we are seeing the background increase recently (solutions have been made up several times - so I do not believe there is a technical error in concentrations.)
Our recipe is:
1000 mL of 1% gelatin, Bloom 275
38 mL of 4% Chrome Alum
Thanks, and have a great weekend.
Mary
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