Gelatin/Chrome Alum for bone

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From:Mary Stevens <>
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Hi all,

For years, I've been using the same Gel-Chrome Alum Concentrations/methods to coat slides for undecalcified bone (for MMA) - lately we are getting an overall background staining on the slides (not clumping, but what appears as too much gelatin.)  The obvious thought is to decrease the 1% to 0.75% gelatin - but I'd like to hear what solutions you are using and your thoughts on why we are seeing the background increase recently (solutions have been made up several times - so I do not believe there is a technical error in concentrations.)

Our recipe is:
1000 mL of 1% gelatin, Bloom 275
38 mL of 4% Chrome Alum

Thanks, and have a great weekend.


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