RE: Oops--wrong 2ndary--any hope?

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From:"Barry, Lilith" <>
To:'Bret Morrow' <>, HistoNet Server <>
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What do you mean by "wrong secondary"?
If your secondary is raised against the animal that the primary was made in,
then you have a problem. If not than you can do your immunoreaction again.
Need more information to help.

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Lilith Ohannessian-Barry                               
National Research Council                          
Institute of Biological Sciences                  

-----Original Message-----
From: Bret Morrow []
Sent: Wednesday, July 12, 2000 12:33 PM
To: HistoNet Server
Subject: Oops--wrong 2ndary--any hope?


We had a SNAFU and probed free-floating tissue with the right primary
but the wrong 2ndary antibody and then ABC.  The tissue was NOT exposed
to DAB chromogen.  Before we figured out what had happened, we washed
the tissue and placed it into a cryo solution and stored it @ -20 oC.
The tissue is reasonable valuable and is, of course, the last set of
tissue slices from these animals.  Now that we know the wrong secondary
was used, what are the odds that we can get any signal from the antigen
target using the right secondary?  Could we re-expose to more primary
and then the right secondary and get a good signal?   Is there concern
that the signal may be diminished?   Any thoughts on this, anyone?
Bret A. Morrow, Ph.D.
Associate research scientist
Associate clinical professor
Yale University School of Medicine
New Haven, CT 06520-8066

voice 203.785.4515
fax  203.785.7670

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