Androgen receptor in rat tissue

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From:"Harclerode, Donna" <> (by way of histonet)
To:histonet <>
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Novacastra (available from Vector in the US) has a polyclonal (catalog
NCL-ARp) that works on rat paraffin sections (on hamster too).  I use it
at 1:40 overnight 4oC  incubation with microwave citrate buffer
pretreatment. I use a labeled strep avidin -HRP system with DAB.  There
is more background than I would prefer but the monoclonals I tried have
not worked.

Donna Harclerode HT (ASCP) HTL, QIHC
Ligand Pharmaceuticals
San Diego, CA

[Harclerode, Donna]  Date: 18 Jan 1999 09:15:34 -0600
From: "Richard   Edwards" <>
Subject: Antibody  search

           HELP PLEASE

Anyone  out  there actually  using an anti-androgen receptor antibody
works on paraffin sections of  rat tissue??????

                                        Richard  Edwards

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