Androgen receptor in rat tissue

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From:"Harclerode, Donna" <DHarclerode@ligand.com> (by way of histonet)
To:histonet <histonet@magicnet.net>
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Richard,
Novacastra (available from Vector in the US) has a polyclonal (catalog
NCL-ARp) that works on rat paraffin sections (on hamster too).  I use it
at 1:40 overnight 4oC  incubation with microwave citrate buffer
pretreatment. I use a labeled strep avidin -HRP system with DAB.  There
is more background than I would prefer but the monoclonals I tried have
not worked.

Donna Harclerode HT (ASCP) HTL, QIHC
Ligand Pharmaceuticals
San Diego, CA


[Harclerode, Donna]  Date: 18 Jan 1999 09:15:34 -0600
From: "Richard   Edwards" <REE3@leicester.ac.uk>
Subject: Antibody  search



           HELP PLEASE

Anyone  out  there actually  using an anti-androgen receptor antibody
that
works on paraffin sections of  rat tissue??????

                              Cheers
                                        Richard  Edwards
                                                               MRC
TOXICOLOGY
	 !




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