Androgen receptor in rat tissue
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From: | "Harclerode, Donna" <DHarclerode@ligand.com> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Richard,
Novacastra (available from Vector in the US) has a polyclonal (catalog
NCL-ARp) that works on rat paraffin sections (on hamster too). I use it
at 1:40 overnight 4oC incubation with microwave citrate buffer
pretreatment. I use a labeled strep avidin -HRP system with DAB. There
is more background than I would prefer but the monoclonals I tried have
not worked.
Donna Harclerode HT (ASCP) HTL, QIHC
Ligand Pharmaceuticals
San Diego, CA
[Harclerode, Donna] Date: 18 Jan 1999 09:15:34 -0600
From: "Richard Edwards" <REE3@leicester.ac.uk>
Subject: Antibody search
HELP PLEASE
Anyone out there actually using an anti-androgen receptor antibody
that
works on paraffin sections of rat tissue??????
Cheers
Richard Edwards
MRC
TOXICOLOGY
!
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