I am having similar problems with frozen sections, bubbling up, looking
strung out across the slide.  Could it be a bad lot of plus charged
slides?  When something like this suddenly happens, and nothing else has
changed in the remainder of the procedure, I get antsy about the slides.
And I do not require any antigen retrieval.

Have changed fixative to fresh, new buffers, etc ad nauseum, and still
have problems.  My usual double acetone fixation protocol, plus long
air drying before fix (longer now!) hasn't improved the problem.

Has anyone else run into bad lots of plus charged slides?  They are usually
the one thing that is truly reliable, but maybe not in this case.

Gayle Callis