RE: Antibody Lot/More About Lot Numbers

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Thanks for the good information.  I wanted to touch on lot numbers a little

Lot numbers are given to batches.  Batch numbers trace back to raw
materials and may trace back to other information such as date of
manufacture and location of manufacturing.  The information that a lot
number contains is dependent on the manufacturer.  In the case of
antibodies,  I would agree with not mixing lots.  The manufacturing
practices, specifications and applications are too delicate.

The lot number is important information to give to the manufacturer along
with a problem.   ISO and GMP facilities have complaint systems in place.
Also, they do tracking of complaints and have to show resolution to
problems.  It's an opportunity for improvement on the manufacturers end and
an opporutnity for end-users to get the products they need to work with.
Manufacturers need to hear complaints (along with the good stuff).

Rande Kline HT (ASCP)
Technical Services
EM Science

Cynthia Favara <> on 01/19/99 09:17:00 AM

To:   "" <>,
      "''" <>
Subject:  RE: Antibody Lot Test Question

     My understanding of lot numbers is that they are  batch numbers.
Large volume that has been aliquot. Therefore I would not mix different lot
numbers. I would also not mix the different samples from the same lot. Each
aliquot has the potential of being subjected to detrimental treatment,
temperature would be an example. I would check a new aliquot of antibody
even with the same lot number for the same reason.
     I view lot numbers as a way of checking with the company if I
happens to have problems at the bench.

Cynthia Favara
Rocky Mountain Laboratories
903 S 4th Street
Hamilton, MT 59840
ph: 406-363-9317
FAX: 406-363-9286

> ----------
> From:[]
> Sent:   Friday, January 15, 1999 9:02 PM
> To:
> Subject:     Antibody Lot Test Question
> Histonetters,
>    A couple of immnu lot testing queries....
> -How do you feel about mixing primary antibody lots together when there
> isn't enough of the old lot.
>  Whether you say "Yay" or "Nay", please include your reasoning.
> -If a new antibody lot arrives in the lab and it has the same lot number
> as the current tested antibody, is it necessary to test it?  Again,
> please share your reasons for agreeing or disagreeing.
> -What DO you do with a drunken sailor?
>                        Many thanks,
>                        Sandosis
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