Re: [Histonet] Fluorescence staining

From:Gayle Callis

Molecular Probes has mounting media designed to set up hard but mainly 
prevent photobleaching of fluorophore, in your case FITC?

Go to their website and look for Prolong Gold, ready to use.  We love 
it.  You mount the sections from PBS, and do not dehydrate.  They recommend 
sealing the edges of coverslip, which is done nicely with toluene or xylene 
diluted mounting media at consistency of very thin nail polish. We avoid 
latter due to alcohol in nail polish.

It is pricey but worth every penny of cost.

At 09:10 AM 1/25/2005, you wrote:
>We are looking into trying some Fluorescence staining. We want to try a 
>TSA Fluorescence System from PerkinElmer. The IHC protocol does however 
>leave me with some questions.
>We always dehydrate our samples after staining. The protocol however 
>doesn't mention this. It only says that the samples can be counterstained 
>and mounted after incubation with the Fluorophore Tyramide. Should we 
>dehydrate and is there a particular mounting media we should use to 
>prolong the time that fluorescence can be detected? Any help would be 
>greatly appreciated.
>Thanks in advance for all your help,
>Georgetown University
>Research Assistant

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)

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