[Histonet] cd15 and morphology on bone marrows and lymph nodes

From:"Van Eyck, Deb"

     Hi all,  
1.I would really appreciate some replies to a couple of questions.  One is
CD15; I thought  I had a pretty good antibody going, but it is still
problematic in some cases.  I would like to know what others are using
consistently with heme path approval.  The same goes for kappa and lambda
and clonality. 

2.  The other issue is handling bone marrows and lymph nodes---we have
recently gotten rid of B-5 and we use NBF as our main fixative.  I would
like some replies in regards to what is the best fixation/decal situation
for morphology purposes (does anyone feel that they have reached the b-5
nirvana state for good morphology) and still maintain immuno staining????
Times and protocols and products would be great.    

Thanks----Deb Van Eyck-Anatomic Path
Waukesha Memorial Hospital
725 American Ave.
Waukesha,WI 53188

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