[Histonet] Mouse Lung inflation-OCT


OCT is almost impossible to use to inflate the lungs, it is very viscous,
but I have used a 1:1 OCT and 5%sucrose solution to inflate adult mouse
lungs and it adds tremendously to the morphology of the frozen lung tissue
and makes it easier to section also. Immunohistochemistry and in situ
procedures have been performed on these samples with no adverse effects.

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