[Histonet] RE: Humanized mouse monoclonals
Dear Tom,
For the detection of a "humanized" monoclonal antibody on human tissue
one need a procedure similar to the ARKit: in vitro labeling of the
primary antibody with a biotinylated goat anti-IgG (of primary
species) Fab-fragment. Such a kit is available from Molecular Probes
and called a Zenon-kit
(http://www.probes.com/products/zenon/zenon.html). According to their
website it is available for mouse, rabbit and human primary antibodies
and the label is either biotin or a fluorochrome.
Chris van der Loos, PhD
Dept. of Pathology
Academical Medical Center M2-230
NL-1105 AZ Amsterdam
The Netherlands
----- Original Message -----
>From Thomas Crowell
Date Tue, 11 Jan 2005 12:41:40 -0500
To histonet@lists.utsouthwestern.edu
Subject [Histonet] Humanized mouse monoclonals
Dear Histonetters,
I was wondering if anyone could provide me with a basic protocol using
a
preformed complex of a humanized mouse monoclonal (human Fc ) with a
biotinylated anti-human IgG to detect antigens in human cryostat
sections
- essentially, human on human IHC staining? I have several antibodies
with different total protein concentrations.
Thanks
Tom Crowell
BiogenIDEC
Cambridge, MA
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