Lesley,
       Have to disagree with you regarding soft water areas. Here in sunny Glasgow the water is as soft as a babies bottom and it blues haematoxylin beautifully. In 36 years I've never used or had to use a tap water substitute. I think  Carolyn has another wee problem.
Ian.

----- Original Message -----
From: "Lesley Weston" <lesley@vancouverbc.net>
To: "Demarinis,Carolyn" <cdemarinis@SARATOGACARE.ORG>; "HISTONET (E-mail)"
<HISTONET@pathology.swmed.edu>
Sent: Tuesday, January 28, 2003 6:54 PM
Subject: Re: Masson Trichrome stain


> You have probably thought of this, but just in case - how are you blueing
> the haematoxylin? If you are in a soft-water area, tap water alone won't
> work; you need to use lithium carbonate or Scott's tap-water substitute,
or
> some other alkaline mix.
>
> Lesley Weston.
>
>
> on 28/01/2003 4:14 AM, Demarinis,Carolyn at cdemarinis@SARATOGACARE.ORG
> wrote:
>
> > Each time we perform a Masson Trichrome stain, the nuclei are bright
red.  I
> > have used Polyscientific and Sigma kits, with both Weigerts and Harris
> > Hematoxylin.  Can anyone offer any suggestions?
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>
Dr. Ian Montgomery,
Histotechnology,
Graham Kerr Building,
Institute of Biomedical & Life Sciences,
University of Glasgow,
Glasgow,
G12 8QQ.
Tel: 0141 339 8855
Office: 4652
Lab: 6644.
Pager: 07625 702883
e-mail: ian.montgomery@bio.gla.ac.uk