Double, triple, and quadruple staining is quite simple to perform with the
various kits out there.  I am currently using DakoCytomation's Envision
Doublestain for a variety of antibodies.  I generally perform a double stain
utilizing DAB and Vector VIP purple with a methyl green counterstain. 
 
The results may be quanitated by any number of image analyzers
(Chromavision, Applied Imaging, etc).
Our lab will do this as a referral test if you are interested.  Please
contact me if you have any questions.
 
Sincerely,
Lori A. Cummings 
Clinical Coordinator, Anatomic Pathology 
University of Illinois Medical Center @ Chicago 
office: 312.413.1607 
fax: 312.413.1346 
page id: 6315 

-----Original Message-----
From: Dmborel@aol.com [mailto:Dmborel@aol.com]
Sent: Wednesday, January 29, 2003 1:59 PM
To: histonet@pathology.swmed.edu
Subject: double label IHC stains for skin biopsies


Does anyone know of a lab which can perform double label IHC stains
involving a different chromagen for each of the two labels: CD4+CD45RO+
(UCHL-1) and CD8+CD45RO+ (UCHL-1) on formalin fixed paraffin embedded tissue
or on frozen tissue?  Is there a way to quantitate these, or must one do a
semiquantitative examination by estimating the percentage of positive cells?

Thanks for your help.  These have been requested as part of a research
project for a drug trial.

David M. Borel, MD
Dermatopathology Diagnostics 

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