Re: circularly polarized light

From:"J. A. Kiernan"

I've looked at such sections. An advantage over plane
polarization is that the collagen fibres don't come and
go as you rotate the stage. A disadvantage is that you
don't get a black background. For an illustrated
account of the method, see this paper:

Whittaker P, Kloner RA, Boughner DR, Pickering JG (1994) 
Quantitative assessment of myocardial collagen with 
picrosirius red staining and circularly polarized 
light. Basic Research in Cardiology 89: 397-410.

Peter Whittaker also wrote a review article about the
technique a year later:
Whittaker P (1995) Polarized light microscopy in 
biomedical research. Microscopy and Analysis 44: 15-17.

John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
______________________________________________ wrote:
> Hi,
> Does anyone have experience with (picro sirius red) analysis using circularly
> polarized light instead of linearly polarized light. The former might solve
> some problems ocurring when using the latter. If anyone does have experience
> with circularly polarized light, can they also tell me how they create this
> light. Help is greatly acknowledged!
> Dolf Segers
> Dept. of Experimental Cardiology
> Thoraxcenter, Erasmus MC, Rotterdam
> The Netherlands

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