sample prep: RE: peripheral blood IHC
To fix the monocytes:
Spin the blood tube so you have a buffy coat.
Carefully draw off most of the serum above the buffy coat (leave about 1mm
above buffy coat).
Carefully add fixative (formalin or gluteraldehyde)so the buffy coat is not
disturbed. Leave overnight.
Next day the fixative can be drawn off and the buffy coat will be a solid
disc which can be removed from the tube in one piece.
Divide the disc to show the cross-section and process as a cell block.
A cross-section of the disc will show the white cells and platelets
stratified according to size.
From: Coskran, Timothy M [mailto:firstname.lastname@example.org]
Sent: Thursday, January 09, 2003 10:03 AM
Subject: peripheral blood IHC
Does anyone have any experience they could share on labeling human monocytes
in peripheral blood? I'm interested in any info you may have on sample prep
and fixation as well as antibody choices and protocols.
Unless expressly stated otherwise, this message is confidential and may be
privileged. It is intended for the addressee(s) only. Access to this E-mail
by anyone else is unauthorized. If you are not an addressee, any disclosure
or copying of the contents of this E-mail or any action taken (or not taken)
in reliance on it is unauthorized and may be unlawful. If you are not an
addressee, please inform the sender immediately.
<< Previous Message | Next Message >>