sample prep: RE: peripheral blood IHC

From:"Morken, Tim"

To fix the monocytes:
Spin the blood tube so you have a buffy coat.
Carefully draw off most of the serum above the buffy coat (leave about 1mm
above buffy coat).
Carefully add fixative (formalin or gluteraldehyde)so the buffy coat is not
disturbed. Leave overnight.
Next day the fixative can be drawn off and the buffy coat will be a solid
disc which can be removed from the tube in one piece. 
Divide the disc to show the cross-section and process as a cell block.
A cross-section of the disc will show the white cells and platelets
stratified according to size.

Tim Morken
CDC, Atlanta

-----Original Message-----
From: Coskran, Timothy M []
Sent: Thursday, January 09, 2003 10:03 AM
To: ''
Subject: peripheral blood IHC


Does anyone have any experience they could share on labeling human monocytes
in peripheral blood?  I'm interested in any info you may have on sample prep
and fixation as well as antibody choices and protocols.

Tim Coskran

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