Cynthia Favara wrote:

> All,
>         I have been asked to stain free floating sections and am enlisting
> my greatest resource. If possible we would like to use frozen tissue and
> thick sections. The premise is that we can stain without the tissue ever
> drying. Advice from anyone with experience would be greatly appreciated.
> I'll take any ideas and promise not to be offended by sarcasm etc [polly
> would definitely freeze here today]
> TIA,

    I stain 20 micron frozen sections of fixed mouse (or rat) brain in 24 well
tissue culture plates. I use 300 microliters per well for 2-3 sections (more
sections if they are small) and do the incubations on a shaker table. If you
put too much solution in the wells you will not get proper agitation. I change
solutions by pipetting, no need to move sections. Working against a black
background makes it much easier to see the sections when changing solutions!

Geoff
--
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff@umdnj.edu
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