Re: free floating sections

<< Previous Message | Next Message >>
From:Geoff McAuliffe <mcauliff@UMDNJ.EDU> (by way of Marvin Hanna)
Content-Type:text/plain; charset="us-ascii"

Cynthia Favara wrote:

> All,
>         I have been asked to stain free floating sections and am enlisting
> my greatest resource. If possible we would like to use frozen tissue and
> thick sections. The premise is that we can stain without the tissue ever
> drying. Advice from anyone with experience would be greatly appreciated.
> I'll take any ideas and promise not to be offended by sarcasm etc [polly
> would definitely freeze here today]
> TIA,

    I stain 20 micron frozen sections of fixed mouse (or rat) brain in 24 well
tissue culture plates. I use 300 microliters per well for 2-3 sections (more
sections if they are small) and do the incubations on a shaker table. If you
put too much solution in the wells you will not get proper agitation. I change
solutions by pipetting, no need to move sections. Working against a black
background makes it much easier to see the sections when changing solutions!

Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029

<< Previous Message | Next Message >>