RE: Gelvatrol

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From:Hewlett Bryan <HEWLETT@HHSC.CA> (by way of Marvin Hanna)
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We haven't used Gelvatol for 20 years, glycerol-gelatin is much cheaper,
easier to make and store. However, if you insist, I've resurrected the
following formulation;

Mix 20g Gelvatol 20/30( Monsanto Chemical Corp., Indian Orchard, MA) in 40mL
glycerol, for 1 hour with constant agitation and gentle heat (50-60C).  Add
80.0mL 0.05 M Tris buffer pH 9.6 which contains Sodium Azide or Thimerosal
as a preservative and Thymol Blue indicator, cover loosely and continue
mixing with agitation and heat for 1 -2 hours. Adjust pH as necessary with
0.05M Tris buffer. Thymol Blue is yellow at pH8.0, green at pH9.0 and blue
at pH9.6. Remove undissolved particles by centrifugation, decant into
airtight glass bottles and store at 4C. Discard any mountant that turns
cloudy, precipitates or polymerizes. Restore pH by adding a few drops of
Tris buffer if needed.


> ----------
> From:[]
> Sent: 	January 26, 2000 2:48 AM
> To:
> Subject: 	Gelvatrol
> Does anyone have a recipe for Gelvatrol mounting medium for
> immunofluorescent
> slides?
> Ellen Yee
> Central Histology Facility
> Sacramento, CA

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