Karen,
We do people tissue, but what really made a difference in our H&E was
"hooping up" our eosin with Phloxine.  If you are interested, I'll look up
the formula for you it was quite simple to do.  (I'm up in the gross romm,
my files are in the basement in EM) We also switched to Richard Allen Hemo,
which was a vast improvement over the shall remain nameless yucky brand we
were using.  let me know if I can help.
Wanda Shotsberger
Harris Methodist Hospital
Fort Worth Texas
 ----------
From: larisonk@uoneuro.uoregon.edu
To: HistoNet@pathology.swmed.edu
Subject: Which H&E?
Date: Monday, January 10, 2000 5:03AM

Hello Netters,

I'm trying to brighten up my H&E stain.  It's been suggested that I switch
from
Gill's #2 hematoxylin to Harris' hematoxylin.  I'm hesitant to do this,
simply
because I'm loathe to use mercuric oxide if it can be avoided.  Will this
change make
a big difference in the quality of my staining?  (I'm staining adult fish).

Also, it's been recommended that I switch to an eosin containing a higher
percentage
of alcohol.  This is no problem, but are there problems I might encounter by
making
this switch?

Karen in Oregon