Re: Which H&E?

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From:"Robbin Newlin Manager, Histology" <> (by way of histonet)
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(Karen Larison) wrote:
> Hello Netters,
> I'm trying to brighten up my H&E stain.  It's been suggested that I
>switch from
> Gill's #2 hematoxylin to Harris' hematoxylin.  I'm hesitant to do this,
> because I'm loathe to use mercuric oxide if it can be avoided.  Will this
>change make
> a big difference in the quality of my staining?  (I'm staining adult fish).
> Also, it's been recommended that I switch to an eosin containing a higher
> of alcohol.  This is no problem, but are there problems I might encounter
>by making
> this switch?
> Karen in Oregon
Hi Karen
I order my hematoxylin and eosin from surgipath. I love the staining
that I get they are always bright and crisp, and thier Harris formula
does not contain mercury.Call them and they may send you a sample to
try. 1-800-678-2216
Good Luck

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