Re: Which H&E?
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From: | "Robbin Newlin Manager, Histology" <rnewlin@burnham-inst.org> (by way of histonet) |
To: | histonet@histosearch.com |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
(Karen Larison) wrote:
>
> Hello Netters,
>
> I'm trying to brighten up my H&E stain. It's been suggested that I
>switch from
> Gill's #2 hematoxylin to Harris' hematoxylin. I'm hesitant to do this,
>simply
> because I'm loathe to use mercuric oxide if it can be avoided. Will this
>change make
> a big difference in the quality of my staining? (I'm staining adult fish).
>
> Also, it's been recommended that I switch to an eosin containing a higher
>percentage
> of alcohol. This is no problem, but are there problems I might encounter
>by making
> this switch?
>
> Karen in Oregon
>
>
Hi Karen
I order my hematoxylin and eosin from surgipath. I love the staining
that I get they are always bright and crisp, and thier Harris formula
does not contain mercury.Call them and they may send you a sample to
try. 1-800-678-2216
Good Luck
Robbin
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