fluorescent bone labels

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From:Gayle Callis <uvsgc@msu.oscs.montana.edu> (by way of histonet)
To:histonet <histonet@magicnet.net>
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This technic will not work with decalcified, paraffin sections since
the fluorecent label (tetracyline dye) will be removed by all the water
in subsequent decalcification, processing.

PMMA is the accepted classic method.  To preserve the label,
alcoholic formalin is an excellent fixative, and NBF has been reported to
work, but the alcohol is the key to help prevent extraction of the
fluorescent marker.  You will get better morphology with alcohol formalin
(Anatech has an excellent one) than fixation with 70% ethanol (the classic
fixation).  I was taught to avoid aqueous fixatives, but some people
seem to have success with NBF.

Gayle Callis

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