RE: [Histonet] frozens on fat


At 1:55 PM +0000 2/6/07, Edwards, R.E. wrote:
>We  have  successfully  cut cryostat  sections  of  mouse  brown  fat,
>using  standard methods, freezing  down  in  dry  ice, embedded  in
>OCT, followed  by  Oil Red O, was  not  a  problem....

I think the secret here is "freezing  down  in  dry  ice" rather than 
using just the cryostat. Freezing in a slurry of isopentane in liquid 
nitrogen also works for hi fat tissues, though that may be overkill 
for adipose tissue. It works wonders for brain FS.

Bill Blank, MD
Heartland Lab

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