We  have  successfully  cut cryostat  sections  of  mouse  brown  fat,
using  standard methods, freezing  down  in  dry  ice, embedded  in
OCT, followed  by  Oil Red O, was  not  a  problem.... 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Derek
Papalegis
Sent: 06 February 2007 13:34
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] frozens on fat

I just got asked today if it is possible to cut frozens on brown fat in
mice. In the past, in a clinical setting, I know when cutting lymph
nodes, we never were able to get any of the fat with them on the slides.
Does anyone know if this is possible to do? If so, what techniques are
you using to get the frozen fat sections? If it is not possible, can
anyone offer any alternatives that I can give to the investigator?

Thanks,

Derek


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