You can usually get this kind of tissue cold enough to section by spraying it thoroughly with one of the refrigerant sprays that most biological supply companies sell. This will bring the tissue down to -50 C. Then take the section quickly, before the block has a chance to start warming up. If you use an anti-roll plate on your cryostat, spray that also.

> ----------
> From: 	histonet-bounces@lists.utsouthwestern.edu on behalf of Derek Papalegis
> Sent: 	Tuesday, February 6, 2007 5:34 AM
> To: 	histonet@lists.utsouthwestern.edu
> Subject: 	[Histonet] frozens on fat
> 
> I just got asked today if it is possible to cut frozens on brown fat in 
> mice. In the past, in a clinical setting, I know when cutting lymph 
> nodes, we never were able to get any of the fat with them on the 
> slides. Does anyone know if this is possible to do? If so, what 
> techniques are you using to get the frozen fat sections? If it is not 
> possible, can anyone offer any alternatives that I can give to the 
> investigator?
> 
> Thanks,
> 
> Derek
> 
> 
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