[Histonet] RE: double immunofluorescent staining
Given that your first and second primary are both mouse antibodies you may try the following:
Normal Mouse serum (1:10, 15 min, do not wash)
CD4/FITC (it's not difficult to purchase this one)
Lots of success with your staining!
Chris van der Loos, PhD
Dept. of Pathology
Academical Medical Center
Amsterdam - The Netherlands
----- Original Message -----
Date Tue, 17 Feb 2004 16:19:17 +0000
>From "Dave McClister"
Subject [Histonet] double immunofluorescent staining
I'm in search of a protocol for double staining.
I'm currrently testing:
First Primary= CD-4
First Secondary= FITC labeled
Second Primary= MMP-8
Second Secondary= Rhodamine
Any help would be greatly appreciated.
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