Dear Dave,
Given that your first and second primary are both mouse antibodies you may try the following:
MMP-8, Ms
anti-Ms/Rhodamine
Normal Mouse serum (1:10, 15 min, do not wash)
CD4/FITC (it's not difficult to purchase this one)
Lots of success with your staining!

Chris van der Loos, PhD
Dept. of Pathology
Academical Medical Center
Amsterdam - The Netherlands


----- Original Message -----
Date  Tue, 17 Feb 2004 16:19:17 +0000 
>From  "Dave McClister" 
To  histonet@lists.utsouthwestern.edu 
Subject  [Histonet] double immunofluorescent staining 
I'm in search of a protocol for double staining.
I'm currrently testing:
First Primary= CD-4
First Secondary= FITC labeled
Second Primary= MMP-8
Second Secondary= Rhodamine

Any help would be greatly appreciated.

Dave McClister
843-789-6826 wk
843-792-0590  pg#1-1274
davenhv@hotmail.com




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