[Histonet] vWf in rat kidneys - the answer
From: | antje.marcantonio@pharma.novartis.com |
Hello Histonetters
vWf staining in kidneys is not a mystery anymore !
Adapting my protocol to the procedure I found in the report mentioned
below , I got wonderful strong staining even in the glomeruli !
Pepsin was the trick ...(Proteinase K didn't help)
So now we run two completely different protocols one for kidneys and one
for lungs.
1: Kidney Int. 2000 Dec;58(6):2599-610.
The vasculopathy of autosomal dominant polycystic kidney disease: insights
from
animal models.
Arnaout MA.
Best regards,
Antje Marcantonio
Novartis Pharma AG
BU Transplantation Research
Basel, Switzerland
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
<< Previous Message | Next Message >>