Nejat,

Collect the blood in a sterile 10ml plain centrifuge tube.
Allow to clot (5-10 minutes should do it)
Centrifuge 10min 1500rpm in a standard centrifuge
Collect supernatant (the serum)

Remember do not add anti-coagulant (eg EDTA, Citrate or Heparin). When you
centrifuge the blood, you will have plasma.

Ask for advice from your Haematology Department.

Regards

Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318




-----Original Message-----
From: Nejat Yilmaz [mailto:nyilmaz@mersin.edu.tr]
Sent: Tuesday, 17 February 2004 9:15 PM
To: histonet
Subject: [Histonet] normal serum preparation


Dear Histonetters...

Does anybody know how can I prepare the normal serum from fresh animal blood
for immunohistochemistry? 

Thanks in advance...
Dr. Necat Yżlmaz
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