on 2/27/01 12:20 PM, Richard Cartun at Rcartun@harthosp.org wrote:

> Has anyone tried doing heat-induced epitope retrieval on de-stained (H&E)
> frozen tissue sections?  I have been asked to stain two frozen section slides,
> one for ER (clone 6F11) and one for TTF-1 (clone 8G7G3/1) because there is no
> tumor left in the permanent sections.  Thanks!
> 
> R. Cartun
> 
> 
I think that both of those antibodies will work on frozen without any
pretreatment. Be sure to run frozen section controls.

P.Loykasek
Phenopath Laboratories
Seattle