Re: HIER on frozen tissue sections
|From:||Patti Loykasek <email@example.com>|
on 2/27/01 12:20 PM, Richard Cartun at Rcartun@harthosp.org wrote:
> Has anyone tried doing heat-induced epitope retrieval on de-stained (H&E)
> frozen tissue sections? I have been asked to stain two frozen section slides,
> one for ER (clone 6F11) and one for TTF-1 (clone 8G7G3/1) because there is no
> tumor left in the permanent sections. Thanks!
> R. Cartun
I think that both of those antibodies will work on frozen without any
pretreatment. Be sure to run frozen section controls.
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