|From:||Tony Henwood <AnthonyH@chw.edu.au>|
Have you tried using an anti-fluorescein antibody followed by your
normal immunohistochemical detection technique. Advantage here is that
you can check the reaction with your UV microscope prior to performing
your IPX reaction. Both monoclonal and polyclonal Abs are available. Try
Beware of using biotinylated probes since if you use an anti-biotin
rather than a direct Avidin demonstration technique, endogenous biotin
will be a problem. It is unmasked after enzyme treatment.
Hope this helps,
Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318
>>> Kappeler Andreas <firstname.lastname@example.org> 26/February/2001
Kreatech in Amsterdam, The Netherlands, has EBER probes labeled with
biotin or digoxigenin (http://www.kreatech.com/). I think Zymed
Kreatech products in the U.S.
Institute of Pathology, University of Bern, Switzerland
From: Rena Fail <email@example.com>
Sent: Sat 24 Feb 2001 02:02
> We are using fluoresceinated Epstein virus EBER probe for in situ
> hybridization and though it is working we would prefer to use a probe
> is not fluoresceinated. Does anyone know of a source for a biotin
> Thank you.
> Rena Fail AS,HT(ASCP)
> SS/IHC lAB
> Medical University of SC
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