HIER on frozen tissue sections

From:Richard Cartun <Rcartun@harthosp.org>

Has anyone tried doing heat-induced epitope retrieval on de-stained (H&E) frozen tissue sections?  I have been asked to stain two frozen section slides, one for ER (clone 6F11) and one for TTF-1 (clone 8G7G3/1) because there is no tumor left in the permanent sections.  Thanks!

R. Cartun

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