Unless the original frozens were fixed in Zamboni's or the 3-step
NBF/methanol/acetone protocol there will probably be no antigen there to
stain. 

Linda A. Sebree, HT(ASCP)
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
A4/204-2472
600 Highland Ave.
Madison, WI 53792-2472
(608)265-6596
FAX: (608)262-7174


-----Original Message-----
From: Richard Cartun [mailto:Rcartun@harthosp.org]
Sent: Tuesday, February 27, 2001 2:21 PM
To: Histonet@pathology.swmed.edu
Subject: HIER on frozen tissue sections


Has anyone tried doing heat-induced epitope retrieval on de-stained (H&E)
frozen tissue sections?  I have been asked to stain two frozen section
slides, one for ER (clone 6F11) and one for TTF-1 (clone 8G7G3/1) because
there is no tumor left in the permanent sections.  Thanks!

R. Cartun