RE: HIER on frozen tissue sections
|From:||"Sebree Linda A." <firstname.lastname@example.org>|
Unless the original frozens were fixed in Zamboni's or the 3-step
NBF/methanol/acetone protocol there will probably be no antigen there to
Linda A. Sebree, HT(ASCP)
University of Wisconsin Hospital & Clinics
600 Highland Ave.
Madison, WI 53792-2472
From: Richard Cartun [mailto:Rcartun@harthosp.org]
Sent: Tuesday, February 27, 2001 2:21 PM
Subject: HIER on frozen tissue sections
Has anyone tried doing heat-induced epitope retrieval on de-stained (H&E)
frozen tissue sections? I have been asked to stain two frozen section
slides, one for ER (clone 6F11) and one for TTF-1 (clone 8G7G3/1) because
there is no tumor left in the permanent sections. Thanks!
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