HistoGel has not had any deleterious effects on IHC with various
pretreatments and microwaving in my experience. Most of the cytoskeletal
proteins, hematopoietic and lymphocytic markers have all expressed similar
consistent results (as to those embedded in paraffin only) with the
HistoGel. Will keep you posted if I find any limitations to using it on IHC!



Eric C. Kellar
University of Pittsburgh Medical Center


	----------
	From: 	Molinari, Betsy[SMTP:BMolinari@heart.thi.tmc.edu]
	Sent: 	Thursday, February 17, 2000 7:37 AM
	To: 	Lynn Gardner; histonet
	Subject: 	RE: Histogel revisited

	Hi Lynn,
	 I originally started the Histogel question, I had a sample and
wanted
	to know what to expect. Since then I have run a test and am really
	pleased with the results. I was able to orientate my teeny tiny
mouse
	carotids in the Histogel,  cool the Histogel,   process it and voila
a
	little cube of Histogel with my carotid perfectly orientated and
ready
	to embed. This is going to save me tons of time, not to mention
	frustration. But I would like to have the same question
answered..does
	Histo gel  effect immuno staining?
	Betsy Molinari


	> ----------
	> From: 	Lynn Gardner[SMTP:lynn-gardner@uiowa.edu]
	> Sent: 	Tuesday, February 15, 2000 11:11 PM
	> To: 	Histonet@pathology.swmed.edu
	> Subject: 	Histogel revisited
	> 
	> Dear all, 
	> 
	> The other day I saw that someone asked if tissue that was in
histogel,
	> fixed and processed could be stained for immunohistochemistry. I
did
	> not
	> see the answers could someone let me know if this is a
possibility?
	> 
	> Thanks all!
	> Lynn Gardner
	> 
	>