Re: Autostainer for IHC

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From:mbpohl <>
To:perl <>
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We have had the Ventana ES since July 1996.  Reliable, easy to use, minimum
maintenance.  Down side:  Can only run at 37 degrees C;  limited to use
their detection kits - and when the price goes up, you feel helpless
(unless you have a reasonable sales person);  in the past little support
from sales rep's --> hopefully I am seeing an improvement there;  limited
technical help -- again, I am told I will see an improvement there;  you
either have to just use antibody dispensers for a run OR you can manually
titrate your own dilutions applying them with a pipette - not both.  I am
told the Ventana Nexus allows both ways to apply antibodies during the same
run - dispenser and manually titrating them.

I will be interested in hearing from others.
perl wrote:

> Dear Histonetters,
> We are in the process of purchasing an Automated
> immunohistochemical stainer.
> If anyone has experience in this field, please let me know which
> system would you recommend.
> Here we can by machines made by: Ventana, Lab Vision, Lleica.
> Samuel
> Dr Samuel Perl
> Head, Dept. of Pathology
> Kimron Veterinary Institute
> P.O.Box 12, 50250 Bet Dagan
> E-mail:  Fax: (972)39681730  Phone: (972) 39681635

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