---------------------- Forwarded by Rande Kline/EMI/Merck on 12/14/99 10:38 AM
---------------------------


Rande Kline
12/14/99 09:12 AM

To:   "Bourassa, Patricia" <patricia_bourassa@groton.pfizer.com>
cc:   "Histonet @Pathology.Swmed.Edu (E-mail)" <histonet@pathology.swmed.edu>
Subject:  Re: Fixation of Smears  (Document link: Database 'Rande Kline', View
      '($Inbox)')

Hi Patty,

You can prepare a blood smear on a regular slide.  You should let the slide
air-dry thoroughly before fixing this will allow the cells to adhere to the
slide and prevent the blood from washing off.  Letting them dry completely is a
very important step.

You haven't mentioned what type of staining you are doing.  If it is a
Romanowsky stain such as Wright or Giemsa than methanol for 30 seconds to 1
minute should work fine.

Rande Kline, HT (ASCP)
Technical Services
EM Science/BDH
An Associate of Merck KGgaA





"Bourassa, Patricia" <patricia_bourassa@groton.pfizer.com> on 12/14/99 07:45:54
AM

To:   "Histonet @Pathology.Swmed.Edu (E-mail)" <histonet@pathology.swmed.edu>
cc:    (bcc: Rande Kline/EMI/Merck)
Subject:  Fixation of Smears



Hi, all!

Not sure if this can be done, but here goes.  We want to make a blood smear
onto a slide and then fix it ASAP.  Can this be done without the smeared
cells falling off the slide?  Thanks!

-Patti Bourassa
 Pfizer, Groton