Penny,
Two references for the technique:

Scaletta, LJ, Occhino, JC, MacCallum, DK, Lillie, JH: "Isolation and
Immunologic Identification of Basement Membrane Zone Antigens From Human
Skin", Lab Invest. 39:1-9, 1978.

Gammon, WR, Briggaman, RA, Inman, AO, Queen, LL, Wheeler, CE.
"Differentiating Anti-Lamina Lucida and Anti-Sublamina Densa Anti-BMZ
Antibodies By Indirect Immunofluorescence On 1.0M Sodium
Chloride-Separated Skin", J. Invest. Dermatol. 82:139-144, 1984.

Bob Santoianni, courtesy of Sue Manos
Emory University Hospital
Atlanta, GA