As far as I know, you CANNOT restain once DAB has been on a tissue.
Mike

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bernice
Frederick
Sent: Friday, December 07, 2007 3:23 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Removing DAB


Question: I need to restain slides for IHC. How do I get rid of DAB
prior to restaining without killing the tissue? Tried a slide I did not
need using DAB-away 1and 2 plus decolorizer (Dako) and yes, it took the
DAB out ,but also took the tissue.

Thanks,

Bernice

 

Bernice Frederick HTL (ASCP)

Northwestern University

Pathology Core Facility

710 N Fairbanks Court

Olson 8-421

Chicago,IL 60611

312-503-3723

 

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