Hi,

We've used a rabbit anti-c Fos antibody from Santa Cruz (SC-52) on rat 
brain tissue that has undergone similar protocol as what you describe.  
The immunofluorescent labelling (visualised with Cy3) worked well for us.

Cheers
Angelina


> Hi there,
>
> one of the user I am working with is having tough time to found a good
> antibody for c-fos to use in cryosection of mouse brain (30micron size) in
> immunofluorescence.
>
> Any suggestion? should she use a different protocol?The protocol right now
> is perfusion with PFA, sucrose, OCT,cutting, permebilization with
> 0.3%triton, blocking with serum and secondary A488.
>
> thanks in advance
>
> Valeria
>
>
>
>
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-- 
~~o~~o~~o~~o~~o~~o~~o~~o~~o~~o

Angelina Y. Fong, Ph.D.
Department of Zoology
Biological Sciences Building
6270 University Boulevard
University of British Columbia
Vancouver, BC, V6T 1Z4
Canada 

Ph:  (604) 822-5990
Fax: (604) 822-2416
Email: fong@zoology.ubc.ca



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