We've used a rabbit anti-c Fos antibody from Santa Cruz (SC-52) on rat
brain tissue that has undergone similar protocol as what you describe.
The immunofluorescent labelling (visualised with Cy3) worked well for us.
> Hi there,
> one of the user I am working with is having tough time to found a good
> antibody for c-fos to use in cryosection of mouse brain (30micron size) in
> Any suggestion? should she use a different protocol?The protocol right now
> is perfusion with PFA, sucrose, OCT,cutting, permebilization with
> 0.3%triton, blocking with serum and secondary A488.
> thanks in advance
> Histonet mailing list
Angelina Y. Fong, Ph.D.
Department of Zoology
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