Once DAB reacts with the Ag-Ab-detection complex, "that's it", it cannot be removed without at least removing the IHC complex.
  René J.

Bernice Frederick  wrote:
  Question: I need to restain slides for IHC. How do I get rid of DAB prior to
restaining without killing the tissue? Tried a slide I did not need using
DAB-away 1and 2 plus decolorizer (Dako) and yes, it took the DAB out ,but
also took the tissue.

Thanks,

Bernice



Bernice Frederick HTL (ASCP)

Northwestern University

Pathology Core Facility

710 N Fairbanks Court

Olson 8-421

Chicago,IL 60611

312-503-3723



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