[Histonet] False positive AFB
From personal experience I do know that acid fast organisms are present in
some tap water. We cultured them from water after some bad experiences with
false positives. Wang reported that somewhere over 30% of water fountains showed
the presence of acid fast organisms (non-pathogens, but who can tell the
difference on a stained slide). This was done using the fluorescence technique.
As a consequence, we filtered our water bath water through a Millipore filter
after it was already passed through a charcoal filter and a deionizing column.
We also used no tap water prior to the carbol-fuchsin and we ran a negative
control (uterus - extra cuts from blocks as the routine H&Es were cut) from
the same days workload with each batch of AFB slides. So take care with your
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