[Histonet] Re: Histonet digest, Vol 1 #167 - 24 msgs

From:tony.j.savage@gsk.com

Nancy,
      In response to message 23 (Vol1 #167 - 24), Why don't you stain with
alcian blue / PAS before you apply your emulsion. Both alcian blue and PAS
should survive subsequent development. I have used this technique in the
past for some of my autoradiographs. You would obviously have to assess
whether or not you are compromising your in situ signal. However I would
have thought you would be on safe ground if you just stained with alcian
blue for a couple of minutes.
                                        Regards,
                                                    Tony
Histopathology Group
Asthma Biology Department.
RIRP CEDD.
GlaxoSmithKline Medicines Research Centre,
Gunnelswood Road,
STEVENAGE,
Hertfordshire.
  SG1 2NY
tel.          +44 (0)1438 764117
fax.         +44 (0)1438 764782
email.    Tony.J.Savage@gsk.com
mobile    +44 07753609835
http://ukdiscovery.gsk.com/histopathology/default.htm




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