From:Garry Ashton

Dear Histonetters,
We are trying to look at GFP on tiny pieces of unfixed tissue using the confocal microscope.
The problem is the tissue has been in culture media which I believe contains methyl red, which is autofluorescing.
Does anybody have any ideas about how to block the autofluorescence without affecting the GFP. I'd assume standard methods such as osmium tetroxide, borohydride etc would also affect the GFP.
Many thanks in advance for any help.

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