Take fresh lung (slightly edematous is better) or fresh placenta with most
of the
blood squeezed/blotted out.

Cut it into 3x3 mm cubes.

Place in appropriate fluid culture media that microbiology has prepared,
with
the micro-organism you want. Gram + in one tube, Gram - in another (I like
E. coli for this one),  AFB in another (non-pathogenic), spirochete in
another,
(non-pathogenic), and fungus in another (haven't tried this yet). Or
whatever
micro-organism you need.

Allow to incubate overnight (37 degree C oven).

Pour on 10% NBF, allow to set 30 minutes, then discard NBF/culture. Then
pour on fresh 10% NBF, and allow to fix completely before processing.

Something else that is easy for Gram +/-, sometimes getting the "extra"
unprocessed pieces of inflammatory appendix works great. Have your
pathologist inform you when he/she sees an appendix with a lot of
inflammation, and grab the unprocessed tissue before it is disposed.

Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak MI 48073

----- Original Message -----
From: "E Sharon Shields" 
To: 
Sent: Friday, December 14, 2001 10:13 AM
Subject: Special Stain Controls


> Does any one have a procedure for making Special Stain controls by
injecting the "bugs" into fresh tissue? Our pathologist have limited the use
of diagnostic tissue blocks for control blocks.  We need to make some
in-house blocks.  The microbiology department will supply the  "bugs" but we
need to get them in the tissue and incubate the tilssue so we get a good
control.  Any ideas?
> Thank you,
> Sharon Shields
> Baptis Hospital of E TN
>
>
>