Sharon,
One easy way is to obtain some fresh placenta, slice
and dice, place in some microbial nutrient media,
innoculate with eg gram positive cocci and gram
negative rods (eg E coli), incubate overnight a/c
microbiology advice, add fixative of choice, process
to wax as desired.
Regards,
Tony (in chef mode - sorry)

--- E Sharon Shields  wrote: >
Does any one have a procedure for making Special
> Stain controls by injecting the "bugs" into fresh
> tissue? Our pathologist have limited the use of
> diagnostic tissue blocks for control blocks.  We
> need to make some in-house blocks.  The microbiology
> department will supply the  "bugs" but we need to
> get them in the tissue and incubate the tilssue so
> we get a good control.  Any ideas?
> Thank you,
> Sharon Shields
> Baptis Hospital of E TN
> 
>  

=====
Tony Henwood JP BAppSc GradDipSysAnaly CT(ASC)
Laboratory Manager
Histopathology Department
The Childrens Hospital at Westmead
Locked Bag 4001
WESTMEAD, 2145
AUSTRALIA

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