Janis,
It seems that the sections were airdried prior to
acetone treatment (the usual). Hxs are notoriously
faint when used on air dried sections. I'd suggest
prolonging the Hx staining time, or possibly using a
different formulation (eg Harris's).
Are you sure that the Mayers works on FFPE sections?
 

--- "Rodgers, Janis"  wrote:
> Histonet question:
> 
> Has anyone had a problem with tissue not picking up
> the Mayer's counterstain
> after doing immunohistochemistry on Frozen sections.
> A lady called me with
> this problem and all I could think of was possibly a
> PH problem. The
> protocol she told me is below
> 
> 1. Acetone 
> 2. Primary O/N
>  Then when she got done with the substrate step she
> fixes in Para+
> Glutaraldehyde. She also left it overnight in buffer
> at one point before she
> finished her procedure. 
> 
> Please send any suggestions  to
> pamela-tompkins@uiowa.edu +
> sally-prouty@uiowa.edu
> 
> Thank You,
>  Sally Prouty
> 
> 
> 
> 
> 
>  

=====
Tony Henwood JP BAppSc GradDipSysAnaly CT(ASC)
Laboratory Manager
Histopathology Department
The Childrens Hospital at Westmead
Locked Bag 4001
WESTMEAD, 2145
AUSTRALIA

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