Re: Bone Marrow
Rita Riddle at Lexington [Kentucky?] Medical Center asks:
>>I need help with Bone Marrow. We have been using something called
Decal-Stat for decaling [sic] bone marrow. Couple of our Dr. would like
better detail in decaling. Could you please tell me what you use to decal
your bone marrow and the process like length of time and etc. We fix them in
something called M-2 fixative.<<
First of all, find out what the products actually are, rather than letting
them hide behind their trade names. What in the world is M-2 fixative?
First of all, marrows need to be properly fixed, at least an hour and
preferably longer. B-5 fixative is preferable, but if it's not practical for
you, use neutral buffered formalin. Most people do use proprietary
decalcifiers such as Decal (whose representative is a frequent contributor to
Histonet) - don't use "decal" as a generic term for decalcifiying agents.
Whatever decalcifier you use, you must remove the tissue from it promptly
when decalcification is finished, or it will damage the tissue. Most
decalcifiers will do a bone marrow core in less than an hour. Return the
decalcified tissue to neutral buffered formalin or to alcohol.
Follow these directions and you'll get a block somebody can cut and stain a
satisfactory section of.
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