Rita,

If your docs want better morphology, I would recommend fixing in bouin's.
Routine histo in my lab uses it on their bone marrow cores and they look
great.  Also, with whatever decal procedure you're using, never
underestimate the power of a good H2O rinse to remove the formic acid before
processing.  If I have time, I will look into the processing/decal times
that they are using.

Good-luck,

Glen A. Dawson  BS, HT & IHC (ASCP)
Lead IHC Technologist
Milwaukee, WI

-----Original Message-----
From: Rita Riddle [mailto:RitaR@lexhealth.org]
Sent: Friday, December 07, 2001 12:19 PM
To: Histology (E-mail)
Subject: Bone Marrow


Hi Everyone,
I need help with Bone Marrow. We have been using something called
Decal-Stat. for decaling bone marrow. Couple of our Dr. would like better
detail in decaling. Could you please tell me what you use to decal your bone
marrow and the process like length of time and etc.  We fix them in
something called M-2 fixative . Thanks for your help.

Rita Riddle
Lexington Medical Center