We microwave the sections in chromic acid for a few seconds.  Saves the
hour's wait.
Andy Shand
----- Original Message -----
From: Greer, Bonnie <Bonnie.Greer@stjude.org>
To: <Kimcatk@aol.com>; <histonet@pathology.swmed.edu>
Sent: Friday, December 08, 2000 3:52 PM
Subject: RE: GMS & Steiner Chapman / Re: Seeking Staining Procedures


> I have done thousands  of gms always use fresh chromic each time for one
> hour...have never heated it...This help to avoid wash off also...plus u go
> to lunch during the time...(:-)
>
> -----Original Message-----
> From: Kimcatk@aol.com [mailto:Kimcatk@aol.com]
> Sent: Thursday, December 07, 2000 12:47 PM
> To: histonet@pathology.swmed.edu
> Subject: GMS & Steiner Chapman / Re: Seeking Staining Procedures
>
>
> Dear HistoNet colleagues,
>      It is great to have HistoNet working again!  Thank you to those of
you
> who responded to my posting.  I got some leads on staining for fungi and
> spirochetes, but I still have some questions.
>      Has anyone done Grocott's Methenamine Silver (GMS) without heating
the
> chromic acid?  I heard that it is possible to oxidize in the chromic acid
> for an hour instead of heating it, but I do not have a procedure.
>      Also, I am looking for a procedure for Steiner Chapman because that
> replaces the uranium/uranyl nitrate with some type of zinc solution.
>      I would be very grateful for any help you could provide.
>
> Sincerely,
>
> Kimberly Atkin HT (ASCP)
> Boston, MA
>
>
> In a message dated Tue, 5 Dec 2000  8:52:00 AM Eastern Standard Time,
> Kimcatk writes:
>
> << Dear HistoNet colleagues,
>
>      I am seeking to replace some staining procedures in my laboratory
with
> NON-microwave methods.  Optimally, I am hoping to find stains that are
free
> of heavy metals or any particularly toxic or dangerous components.  I can
> not use mercury.
>
>      I need to find staining protocols or kits to demonstrate the
following:
>
> Fungal organisms
> Spirochetes, Campylobacter, and Legionella organisms
>
>      In addition, the gram stain procedure I use is very cumbersome and I
> would be extremely grateful for a simpler method.
>
>      Thank you all very much in advance for your help.
>
> Sincerely,
>
> Kimberly Atkin HT (ASCP)
>
> Boston, Massachusetts
>
>  >>
>
>
>