Tammaryn
We have a small bench top Tutnauer autoclave which we use for 
any of our heat antigen retrieval methods.  I find it easier to use then 
water baths-similar to a pressure cooker.  You have to do a little 
playing at first to get the timing right, but once that is done it works 
well. I find that a setting of 22 minutes on the autoclave works well 
for me.

Mark


> 
> Date: 8 Dec 2000 11:16:56 -0600
> From: Tammaryn Lashley <t.lashley@ion.ucl.ac.uk>
> Subject: Antigen retreival
> 
> Dear All,
> 
> Does anybody out there have any experience with using an autoclave as a
> means of antigen retrieval in fixed tissue?
> 
> Tammaryn
> Dep. of Neuropathology
> Institute of Neurology
> Queens Square House
> Queens Square
> London WC1N 3BG
> 
> Tel: 0171 837 3611 ext 4247
> 
> 
> 

W. Mark Elliott, PhD
Research Associate
Pulmonary Research Laboratory
Room 292, St. Paul's Hospital
1081 Burrard Street
Vancouver BC Canada
V6Z 1Y6

604-806-8346
604-806-8351 (Fax)