RE: GMS & Steiner Chapman / Re: Seeking Staining Procedures

From:"Weems, Joyce" <JWEEMS@sjha.org>

We use 10% - skip the sodium bisulphite - do the silver, gold chloride, and
thio - done in a flash - and just as good as with all the steps!
Joyce Weems
Pathology Manager
Saint Joseph's Hospital of Atlanta


	-----Original Message-----
	From:	Bryan Llewellyn [SMTP:bryand@netbistro.com]
	Sent:	Friday, December 08, 2000 7:58 PM
	To:	Histonet
	Subject:	Re: GMS & Steiner Chapman / Re: Seeking Staining
Procedures

	We use 10% for 10 minutes as well, but after bleaching in sodium
bisulphite
	we place in 0.5% thiosemicarbazide for 5 minutes.  This has an
aldehyde
	blocking group at one end of its molecule and a very strong reducing
agent
	at the other, stronger than the aldehyde.  The result is a faster
and more
	contrasty impregnation in fungus staining as well as BMs.

	Hayashi, I., Tome, Y. and Shimosato, Y., 1989.
	Thiosemicarbazide used after periodic acid makes methenamine silver
staining
	of renal glomerular basement membranes faster and cleaner.
	Stain Technology 64: 185-190.

	Bryan Llewellyn

	----- Original Message -----
	From: "Lee & Peggy Wenk" <lpwenk@mail.netquest.com>
	To: <Kimcatk@aol.com>; <histonet@pathology.swmed.edu>
	Sent: December 8, 2000 2:26 AM
	Subject: Re: GMS & Steiner Chapman / Re: Seeking Staining Procedures


	> We use 10% chromic acid (aqueous) for 10 minutes, room
temperature.
	> We used to use 4% for 1 hour, room temp. But the 10% for 10 min.
gave
	> us the same results and saved us 50 minutes. This solution can be
reused
	> for months. When it starts to turn brown, instead of being orange,
it's
	> time to remake the solution.
	>
	> We tried the 1 minute microwave with the 4%, but we never liked
it. The
	> tissues tended to fall off the slides, and we found that the
solution
	would
	> turn brown after microwaving just a couple of times so we were
making
	> new solution every week or so.
	>
	> Peggy A. Wenk, HTL(ASCP)
	> William Beaumont Hospital
	> Royal Oak, MI
	>
	> ----- Original Message -----
	> From: <Kimcatk@aol.com>
	> To: <histonet@pathology.swmed.edu>
	> Sent: Thursday, December 07, 2000 1:47 PM
	> Subject: GMS & Steiner Chapman / Re: Seeking Staining Procedures
	>
	>
	> > Dear HistoNet colleagues,
	> >      It is great to have HistoNet working again!  Thank you to
those of
	> you who responded to my posting.  I got some leads on staining for
fungi
	and
	> spirochetes, but I still have some questions.
	> >      Has anyone done Grocott's Methenamine Silver (GMS) without
heating
	> the chromic acid?  I heard that it is possible to oxidize in the
chromic
	> acid for an hour instead of heating it, but I do not have a
procedure.
	> >      Also, I am looking for a procedure for Steiner Chapman
because that
	> replaces the uranium/uranyl nitrate with some type of zinc
solution.
	> >      I would be very grateful for any help you could provide.
	> >
	> > Sincerely,
	> >
	> > Kimberly Atkin HT (ASCP)
	> > Boston, MA
	> >
	> >
	> > In a message dated Tue, 5 Dec 2000  8:52:00 AM Eastern Standard
Time,
	> Kimcatk writes:
	> >
	> > << Dear HistoNet colleagues,
	> >
	> >      I am seeking to replace some staining procedures in my
laboratory
	> with NON-microwave methods.  Optimally, I am hoping to find stains
that
	are
	> free of heavy metals or any particularly toxic or dangerous
components.  I
	> can not use mercury.
	> >
	> >      I need to find staining protocols or kits to demonstrate
the
	> following:
	> >
	> > Fungal organisms
	> > Spirochetes, Campylobacter, and Legionella organisms
	> >
	> >      In addition, the gram stain procedure I use is very
cumbersome and
	I
	> would be extremely grateful for a simpler method.
	> >
	> >      Thank you all very much in advance for your help.
	> >
	> > Sincerely,
	> >
	> > Kimberly Atkin HT (ASCP)
	> >
	> > Boston, Massachusetts
	> >
	> >  >>
	> >
	> >
	> >
	> >
	>
	>
	>
	



<< Previous Message | Next Message >>