We use 1:50 with Dako's regular Target Retrieval and Envision +.

Ross Stapf
Histology Supervisor
Washington Adventist Hospital
Takoma Park MD

>>> "THERESA ROHR"  08/07/03 04:19PM
>>>
Hi,

I am having some trouble getting a good dilution on E-Cadherin from
Dako. I 
am using the Envision+ detection system with 20 minutes in Citrate
Antigen 
Retrieval. If anybody is using this antibody, could you let me know
what is 
working for you?

Dako seems to think I should be OK at a range of 1:200 to 1: 600.  It's
just 
not ideal. I even tried a lower dilution and am not really happy. Also

played with Protein Block and longer incubation time, but really I am a

small hospital not a research center. There's just no time for this in
the 
middle of vacations.

We're looking for a new control block but I thought I would put it out
to 
you and see if anyone else had a problem.

Thanks so much,
Theresa Rohr, Section Head, Histology
Nyack Hospital, New York
trohratnyackhospital@hotmail.com 

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