Titebond is an aliphatic resin wood glue.  I would think any yellow
carpenter's woodworking glue would work.  I use Titebond or Elmer's in my
shop and they seem to be about the same.


David Pizi >






-----Original Message-----
From:   louise renton [SMTP:louise_renton@hotmail.com]
Sent:   Friday, August 08, 2003 3:37 AM
To:     funderwood@mcohio.org; histonet@pathology.swmed.edu
Subject:        RE: coated slides Elmer's


Dear Fred,


Sadly, titebond is not available in South Africa. I have made extensive
enquiries, but with no luck. So unless the formula is available (nothing on
the Franklin website) I can't even guess for a substitute.


Thanks anyway




Louise Renton
Bone Research Unit
MRC
Johannesburg
South Africa
Tel & fax +27 11 717 2298
"Time flies like an arrow, fruit flies like a banana"



Dear Fred,


sa



----


Hi Louise.  I use a product called titebond.  It is a water
resistant wood glue.  If you are unable to find that brand I'm sure
that other wood glues would work.  I make an aqueous solution of 1%-5%
(it is thick and difficult to measure an exact amount) and add a grain
of thymol for preservative.  I spread a small drop on a slide and
allow it to dry before mounting the section.  It does a great job of
adhereing dense bone.  Unlike Elmers glue or gelatin, there is no
background staining.


Fred Underwood
Montgomery County Coroner
Dayton, Ohio
Subject: Re: coated slides Elmer's




        Dear all


        I have heard a lot about Elmer's Glue, which I gather is some
type of
household vinyl based adhesive. We don't get this specific brand, but
similar ones,  used specifically for woodwork or crafts are
available.
So...my question is this: How is it used?  Neat, Diluted, in the
waterbath???  PLease advise as I have some deadful whole paw sections
that I
am having difficuly "sticking" to the slide.


        BTW, I have tried chrome alum, but get horrible background so my
thanks go
to Gayle for suggesting placing them in NBF. I will try that too.


        Best regards




        Louise Renton
Bone Research Unit
MRC
Johannesburg
South Africa
Tel & fax +27 11 717 2298
"Time flies like an arrow, fruit flies like a banana"






            At 08:48 a.m. 01/08/2003  0400, you wrote:
 >Hi,
 >I am having problems with tissue staying on my slides after drying
 >overnight
 >in 37C oven and then one more night in a 60C oven.  The tissue is
chicken
 >leg knee joints.   They are formalin fixed, EDTA decaled, and
paraffin
 >embedded samples.  I think that coated slides may help.  Does
anyone have a
 >simple protocol for making coated slides to help this tissue stick?
 >Thanks in advance for the help.
 >
 >Loralee Gehan
 >University of Rochester


        >   Loralle:
You  can  use  a  vinyl glue  like  Elmer#180#s, it
works  fine  in  cases
like you  are  describiyng. The  adhesion  properties  are
at  least
the  same  that  charged slides  when  you are  treating
samples
like  cartilage  and bone.
Good luck,Carlos.


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